| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Poplar Bluff High School | A | Hicks | Sisk | Larkins | Burton | 9:00.85 | Verified |
| 3 | Webster Groves High School | A | Bacon | Richardson | Lack | Ahrens | 8:24.49 | Verified |
| 4 | St. Louis University High School | A | Seal | Brawley | Kellogg | Labarge | 8:10.85 | Verified |
| 5 | Jackson High School | A | Puls | McCadams | Brazel | Gentry | 8:12.51 | Verified |
| 6 | Lindbergh High School | A | Baker | Myers | Liebetreu | Fletcher | 8:27.89 | Verified |
| 7 | Seckman High School | A | Matus | House | Foster | Emery | 8:56.29 | Verified |
| 8 | Mehlville High School | A | Jones | Ramos | Utsey | Nasufovic | 9:14.77 | Verified |
