| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Fox High School | A | Nguyen | Jost | Kirkpatrick | Pisoni | 3:47.98 | Verified |
| 2 | Poplar Bluff High School | A | Persons | Rogers | McFarland | nunn | 3:36.29 | Verified |
| 3 | Seckman High School | A | Gossett | Tilley | Key | Rudanovich | 3:33.53 | Verified |
| 4 | Webster Groves High School | A | Bacon | Lack | Jones | Richardson | 3:27.43 | Verified |
| 5 | St. Louis University High School | A | Parker | Lowe | Seal | Mayer | 3:30.60 | Verified |
| 6 | Lindbergh High School | A | Deterding | Fletcher | Eaton | Baker | 3:35.52 | Verified |
| 7 | Jackson High School | A | Gentry | Puls | CAIN | Alspaugh | 3:36.65 | Verified |
| 8 | Mehlville High School | A | Ordaz | Londal | Pesselato | Jones | 3:57.85 | Verified |
