| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lindbergh High School | A | Balbo | Earley | Glaser | Goss | 46.31 | Verified |
| 2 | Webster Groves High School | A | Miller | Logan | Miller | Casseus | 45.40 | Verified |
| 3 | Mehlville High School | A | Jones | Clayton | DUBOSE | OHMER | 45.00 | Verified |
| 4 | St. Louis University High School | A | Harris | Wingo | Bailey | Oliphant | 42.02 | Verified |
| 5 | Poplar Bluff High School | A | Barousse | mitchell | Gatewood | Hoss | 42.98 | Verified |
| 6 | Seckman High School | A | Borawski | Rellihan | Harp | Horton | 45.30 | Verified |
| 7 | Jackson High School | A | Alley | STRICKLAND | Settle | Wahlers | 46.09 | Verified |
| 8 | Fox High School | A | Nguyen | Gast | Kirkpatrick | Murray | 46.77 | Verified |
