| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MARCUM, Isabella | JR | Fox High School | 5:09.64 | Verified |
| 2 | FLIEGE, Layla | SR | Jackson High School | 5:19.01 | Verified |
| 3 | ANTHONIES, Courtney | SR | Seckman High School | 5:36.63 | Verified |
| 4 | STAAB, Sarah | SO | Webster Groves High School | 5:39.19 | Verified |
| 5 | WELCH, Nora | SR | Webster Groves High School | 5:39.63 | Verified |
| 6 | BURGESS, Brooklyn | SR | Jackson High School | 5:44.76 | Verified |
| 7 | ALMAMORI, Fatimah | JR | Lindbergh High School | 5:57.63 | Verified |
| 8 | MASON, Lauryn | SR | Fox High School | 5:59.29 | Verified |
| 9 | DOSS, Elyse | SO | Lindbergh High School | 6:13.91 | Verified |
| 10 | KIMBERLIN, Mairead | SR | Mehlville High School | 6:14.45 | Verified |
| 11 | LEACH, Sophia | SO | Poplar Bluff High School | 6:14.52 | Verified |
| 12 | ESCAMILLA, Amy | SO | Mehlville High School | NT | Unverified: |
