| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Mid-Buchanan High School | A | Cooper | Brown | Weston | Callaway | 4:02.29 | Verified |
| 4 | Penney High School | A | Ragle | McBee | England | Youtsey | 3:56.66 | Verified |
| 6 | Polo High School | A | Carter | Claypole | Roberts | Owens | 4:04.68 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Maysville High School | A | HULETT | KEESAMAN | Gabbard | Israel | 3:54.62 | Verified |
| 2 | Lathrop High School | A | Adwell | Davis | Quinn | Miller | 3:46.43 | Verified |
| 3 | Lawson HS | A | Brightman | Stephan | Sharp | Vaughn | 3:41.04 | Verified |
| 4 | West Platte High School | A | Hill | Forti | Williams | Kinslow | 3:38.78 | Verified |
| 5 | Plattsburg High School | A | Stockdale | Houser | Lee | Halsey | 3:39.97 | Verified |
| 6 | Lexington High School | A | Day | Christian | Moss | Worthington | 3:41.43 | Verified |
| 7 | Bishop LeBlond High School | A | Waterman | Lile | Ellsworth | Cross | 3:49.62 | Verified |
| 8 | East Buchanan High School | A | Conroy | Paxton | Watkins | Thomas | 3:56.30 | Verified |
