| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | MORELOCK, Katelyn | SO | North Platte High School | 1:06.25 | Verified |
| 4 | GABRIELLI, Valorie | JR | Polo High School | 1:01.37 | Verified |
| 5 | WATERS, Mylee | 9 | Penney High School | 1:05.14 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | ROUSH, Olivia | SO | Lexington High School | 1:00.61 | Verified |
| 3 | PFISTER, Grace | SO | Bishop LeBlond High School | 56.81 | Verified |
| 4 | SEELEY, Kara | SO | Lathrop High School | 53.59 | Verified |
| 5 | MCGARRY, Addison | SO | Mid-Buchanan High School | 53.76 | Verified |
| 6 | MATSUKAVICH, Loralie | 9 | Plattsburg High School | 56.97 | Verified |
| 7 | LAGESCHULTE, Jorja | 9 | Bishop LeBlond High School | 1:01.25 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BEANE, Keylie | SO | Lathrop High School | 53.12 | Verified |
| 2 | CANNON, KYLEE | SO | South Harrison High School | 51.95 | Verified |
| 3 | PETERS, Hailey | 9 | West Platte High School | 50.45 | Verified |
| 4 | REDMAN, Mirabella | JR | Maysville High School | 47.12 | Verified |
| 5 | GILLIAM, Olivia | SR | Lawson HS | 48.98 | Verified |
| 6 | BALL, Annabelle | SO | Gallatin High School | 50.59 | Verified |
| 7 | SUMMERS, Kenedie | SO | North Platte High School | 53.06 | Verified |
| 8 | BARKER, Joelle | 9 | Lawson HS | 53.34 | Verified |
