| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | STRAUS, Delaney | SR | Marion County High School | 2:20.73 | Verified |
| 2 | SHARP, Alex | SR | Brookfield High School | 2:22.30 | Verified |
| 3 | WILLIAMS, Emmalee | SR | Monroe City High School | 2:22.80 | Verified |
| 5 | SHEPUTIS, Laurin | SO | Palmyra High School | 2:40.66 | Verified |
| 6 | HAYS, Meghan | 9 | Monroe City High School | 2:42.69 | Unverified: Monroe City Open #1 |
| 7 | SHARP, Samantha | 9 | Brookfield High School | 2:47.87 | Verified |
| 8 | SMITH, Carlee | SO | Kirksville High School | 2:49.38 | Verified |
| 9 | LUNSFORD, Madison | SR | Green City High School | 2:49.80 | Verified |
| 10 | HARSHBERGER, Emma | SO | Highland High School | 2:52.79 | Verified |
| 11 | HESS, Delana | SR | Clark County High School | 2:54.20 | Verified |
| 12 | JOHNSON, Avery | 9 | Monroe City High School | 2:56.31 | Verified |
| 13 | LEE, Alexa | 9 | Green City High School | 3:01.43 | Verified |
| 14 | JEFFRIES, Britney | SO | Green City High School | 3:03.66 | Verified |
| 15 | LITTLE, TRINITY | 9 | Clark County High School | 3:04.55 | Verified |
| 16 | SPEAK, Jordan | SO | Kirksville High School | 3:33.90 | Verified |
| 17 | CLAY, Araya | 9 | Kirksville High School | NT | Unverified: |
| 18 | GILL, Somer | SO | South Shelby High School | NT | Unverified: |
| 19 | HODGES, Makenna | 9 | Clark County High School | NT |
