| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | SWAIM, Ella | SO | Highland High School | NT | Unverified: |
| 4 | ROBINSON, Caelin | JR | Scotland County High School | 1:16.09 | Verified |
| 5 | YATES, KAYLIE | 9 | Clark County High School | NT | Unverified: |
| 6 | GOINGS, EMERY | 9 | Clark County High School | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WOOD, Clara | SO | South Shelby High School | 1:15.32 | Verified |
| 2 | LEWIS, Austin | 9 | Brookfield High School | 1:12.00 | Unverified: |
| 3 | LEE, Alexa | 9 | Green City High School | 1:11.82 | Verified |
| 4 | KING, Kylin | 9 | Brookfield High School | 1:11.51 | Verified |
| 5 | WALKER, Madison | SO | South Shelby High School | 1:11.67 | Verified |
| 6 | MCNEELY, Ellen | SO | Kirksville High School | 1:11.83 | Verified |
| 7 | PEARL, Carley | SO | Clark County High School | 1:12.03 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CLARK, Jaelynn | SO | Monroe City High School | 1:08.93 | Verified |
| 2 | THOMAS, Maya | JR | Kirksville High School | 1:08.18 | Verified |
| 3 | LOMAN, Alaina | SO | Palmyra High School | 1:05.99 | Verified |
| 4 | STRAUS, Delaney | SR | Marion County High School | 59.73 | Verified |
| 5 | SMITH, Abigail | SO | Monroe City High School | 1:05.95 | Verified |
| 6 | COMPTON, Sydney | 9 | Palmyra High School | 1:07.12 | Unverified: Quincy Triangular |
| 7 | WENNEKER, Ella | SR | Highland High School | 1:08.92 | Verified |
| 8 | SMALL, Shantel | SR | Scotland County High School | 1:09.50 | Verified |
