| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Clark County High School | A | SHINN | DAVIDSON | WHEELER | HAMNER | 51.06 | Verified |
| 4 | Kirksville High School | A | Mendez | Austic | Novencido | Omehele | 49.18 | Verified |
| 5 | Palmyra High School | A | Getz | Johnson | McKeown | Dames | 50.58 | Verified |
| 6 | Clark County High School | B | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Keokuk High School | A | Glasgow | Derr | Eckland | Swainey | 48.66 | Unverified: Falcon Relays |
| 3 | Green City High School | A | Fields | Salas | Forest | Peavler | 47.91 | Verified |
| 4 | Highland High School | A | Bringer | Goehl | Holder | Mallett | 46.08 | Verified |
| 5 | Monroe City High School | A | Jones | Martinez | Talton | Pennewell | 46.96 | Verified |
| 6 | Brookfield High School | A | Boley | Buckner | Carter | Polley | 48.26 | Verified |
| 7 | South Shelby High School | A | Moellering | Owens | Wiseman | Hammond | 48.97 | Verified |
