| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JUETTE, Caleb | SR | Palmyra High School | 5:02.40 | Verified |
| 2 | HARLAN, Kabott | SR | Monroe City High School | 5:06.01 | Unverified: Monroe City HS Open |
| 3 | KREPPS, Prophet | SR | Kirksville High School | 5:10.50 | Verified |
| 4 | NELSON, Ethan | SR | Clark County High School | 5:12.03 | Verified |
| 5 | JENNINGS, Patrick | SR | Kirksville High School | 5:13.79 | Verified |
| 6 | MARLIN, Jack | SR | Kirksville High School | 5:19.37 | Verified |
| 7 | BLAINE, Daytona | SR | South Shelby High School | 5:21.51 | Verified |
| 8 | SHAFFER, Quinton | JR | Scotland County High School | 5:21.93 | Verified |
| 9 | BALDWIN, Alex | 9 | Brookfield High School | 5:29.66 | Verified |
| 10 | WRIGHT, Levi | 9 | Marion County High School | 5:35.00 | Unverified: |
| 11 | WHEELER, Jackson | JR | Monroe City High School | 5:37.23 | Unverified: Monroe City HS Open |
| 12 | BRITT, Porter | 9 | Marion County High School | 5:39.00 | Verified |
| 14 | CLOW, Ethan | SO | Highland High School | 5:52.00 | Verified |
| 15 | MORALES-ESPIAU, Louis | SO | Brookfield High School | 6:18.69 | Verified |
| 16 | RANVILLE, Preston | SR | Canton High School | 6:23.00 | Verified |
| 17 | GIBBS, Elijah | SO | Keokuk High School | 6:41.12 | Unverified: Falcon Relays |
| 18 | ISON, Braden | JR | Keokuk High School | 7:00.00 | Unverified: |
| 19 | SNYDER, Dakota | SR | Palmyra High School | NT | Unverified: |
| 20 | MOORE, Titus | 9 | South Shelby High School | NT | Unverified: |
