| PL | Athlete | Yr. | Team | Time | Pts | HPL |
|---|---|---|---|---|---|---|
| 1 | HERBST, Jersey | SO | Kirksville High School | 49.79 | 10 | 2 (1) |
| 2 | YOUNGBLOOD, Carly | SR | Monroe City High School | 52.19 | 8 | 2 (2) |
| 3 | BRINGER, Ansley | JR | Highland High School | 52.77 | 6 | 2 (3) |
| 4 | VOSS, Tenlee | JR | Palmyra High School | 53.28 | 4 | 2 (4) |
| 5 | LYONS, Dawsyn | JR | Kirksville High School | 53.56 | 2 | 1 (1) |
| 6 | ELEAZARRARAZ, Sofia | 9 | South Shelby High School | 53.60 | 1 | 1 (2) |
| 7 | FOSS, Gracee | 9 | Monroe City High School | 54.39 | 1 (3) | |
| 8 | IZARD, Darcy | JR | Brookfield High School | 55.57 | 2 (5) | |
| 9 | KRIGBAUM, Emma | JR | Palmyra High School | 56.31 | 2 (6) | |
| 10 | STEWART, Layonna | 9 | Keokuk High School | 56.39 | 1 (4) | |
| 11 | BISHOP, Paige | JR | Scotland County High School | 56.65 | 2 (7) | |
| 12 | KELLER, Jenna | SO | Brookfield High School | 57.14 | 2 (8) | |
| 13 | SMALL, Shantel | SR | Scotland County High School | 1:01.82 | 1 (5) | |
| 14 | NEVES, LUNDYN | SO | Clark County High School | 1:02.63 | 1 (6) | |
| 15 | GOBART, Lily | JR | Keokuk High School | 1:03.54 | 1 (7) | |
| HOLLENDER, Brinley | SO | Clark County High School | NT |
