| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Palmyra MS | A | Harper | Kroeger | McNeff | Snow | NT |
| 2 | Clark County RED B | A | Webster | Waples | Davis | Billings | NT |
| 3 | Kirksville Middle School | A | Ihnat | Lee | Lunsford | Abdoulmadjid | NT |
| 4 | Clark County RED | A | Randle | Weaver | Miller | Steele | 2:09.00 |
| 5 | Scotland County MS | A | Hatfield | Campbell | Small | Jacobs | 2:22.00 |
| 6 | Holy Rosary Middle School | A | Beaver | Hays | DeGrave | Chapman | NT |
| 7 | Hannibal Middle School | A | NT | ||||
| 8 | Knox County Junior High | A | Henry | Givens | Reel | Campbell | NT |
