| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Knox County Junior High | A | Lay | Myers | Corbin | Mauck | NT |
| 2 | Kirksville Middle School | A | Guilavogui | Nugent | Kiese | Eitel | NT |
| 3 | Scotland County MS | A | McKinney | Soltis | Frederick | Justice | 2:25.00 |
| 4 | Hannibal Middle School | A | 2:05.00 | ||||
| 5 | Palmyra MS | A | Anderson | Hudson | Wilt | Leon | 2:13.00 |
| 6 | Clark County RED | A | Wheeler | West | McDowell | Pitford | NT |
| 7 | Holy Rosary Middle School | A | Hays | Potterfield | Benson | Willingham | NT |
| 8 | Canton MS | A | Hinton | Taylor | Slater | Brewer | NT |
