| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Highland MS | A | Reed | Speer | Logsdon | Crist | 5:28.31 |
| 2 | Hannibal Middle School | A | 5:14.01 | ||||
| 3 | Palmyra MS | A | James | Leon | Bode | Triplett | 4:56.00 |
| 4 | Kirksville Middle School | A | Pinson | Griner | DeLeeuw | Vice | 4:44.30 |
| 5 | Holy Rosary Middle School | A | Hays | Oswald | Jones | Potterfield | 4:51.63 |
| 6 | Clark County RED | A | Little | Cochenour | Zinnert | Baugh | 5:11.36 |
| 7 | Knox County Junior High | A | Talbot | Strong | Penn | Parsons | 5:14.60 |
| 8 | Canton MS | A | Hudnut | Lewis | McReynolds | Slater | 5:35.34 |
