| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Clark County RED B | A | Perry | Heinze | Ball | Oilar | 1:04.01 |
| 4 | Knox County Junior High | A | Cahalan | Mauck | Miller | Miller | 1:01.44 |
| 5 | Clark County RED | A | Zinnert | Cochenour | Lovell | McDowell | 1:04.01 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Kirksville Middle School | A | Nieters | Kiese | Crawford | Guilavogui | 1:00.71 |
| 2 | Palmyra MS | A | Anderson | James | Ramey | Wilt | 59.42 |
| 3 | Holy Rosary Middle School | A | Jones | Potterfield | Benson | Oswald | 59.29 |
| 4 | Highland MS | A | Murphy | Abell | Rothweiler | Ritterbusch | 56.32 |
| 5 | Canton MS | A | Hudnut | Uhlmeyer | Clay | Oliver | 58.86 |
| 6 | Hannibal Middle School | A | 59.40 | ||||
| 7 | Scotland County MS | A | McKinney | Soltis | Frederick | Holland | 1:00.00 |
