| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Battle High School | A | Anderson | Owusu | Green | Karara | 3:45.44 | Verified |
| 2 | Smith-Cotton High School | A | Sims | Neth | Brewer | Lorenzo | 3:44.94 | Verified |
| 3 | Helias Catholic High School | A | Fisher | Voss | Wilson | Malmstrom | 3:29.20 | Verified |
| 4 | Rock Bridge High School | A | Jokerst | Kemey | Welty | Fletcher | 3:25.63 | Verified |
| 5 | Capital City High School | A | Harrison | Hull | Dyer | Grieve | 3:26.02 | Verified |
| 6 | Hickman High School | A | Holt | Keys | McNally | Zink | 3:37.81 | Verified |
| 7 | Jefferson City High School | A | Wilson | Louraine | Burns | Ice | 3:45.10 | Verified |
