| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Jefferson City High School | A | Jordan | Oliver | Rukaria | Mosley | 3:53.00 | Unverified: |
| 2 | Battle High School | A | Nelson | Harris | Alderton | Lakes | 3:48.00 | Unverified: Darwin Rold Invitational |
| 3 | Smith-Cotton High School | A | Washington | Allen | Blas | Brenich | 3:44.94 | Verified |
| 4 | Capital City High School | A | McNeil | Callison | Barnes | Huggins | 3:26.02 | Verified |
| 5 | Hickman High School | A | Brinkman | Calle | Gould | Harding | 3:39.78 | Unverified: |
| 6 | Rock Bridge High School | A | Bailey | Rychnovsky | Smith | Chinikoski | 3:45.20 | Unverified: |
| 7 | Helias Catholic High School | A | Ammons | Crisostomo | Loesch | Schmidt | 3:49.20 | Unverified: |
