| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Smith-Cotton High School | A | Goodwin | Kensinger | Kuehner | Newkirk | 4:43.42 | Verified |
| 2 | Capital City High School | A | Abbott | Hess | Pistel | Koopman | 4:28.85 | Verified |
| 3 | Jefferson City High School | A | Morris | Theroff | Kinsey | Schonhardt | 4:17.36 | Verified |
| 4 | Rock Bridge High School | A | Parsons | Miller | Steuber | Ford | 4:11.29 | Verified |
| 5 | Hickman High School | A | Orr | Smith | Leigh | Stemmons | 4:16.35 | Verified |
| 6 | Battle High School | A | Ferguson | Butler | Anye | Schultz | 4:25.69 | Verified |
| 7 | Helias Catholic High School | A | Luebbert | Verslues | Morrisey | Harrison | 4:32.20 | Verified |
