| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | TAYLOR, Donivan | 9 | Hickman High School | 24.88 | Verified |
| 3 | OWUSU, Pius | JR | Battle High School | 24.61 | Verified |
| 4 | MILO, Tionne | SO | Hickman High School | 24.24 | Verified |
| 5 | KNERNSCHIELD, Harrison | SO | Helias Catholic High School | 24.27 | Verified |
| 6 | ANDERSON, Jeremiah | 9 | Battle High School | 24.78 | Verified |
| 7 | NEUENSWANDER, Henry | JR | Helias Catholic High School | 25.31 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BORDE-KOUFIE, Emmanuel | JR | Capital City High School | 23.40 | Verified |
| 2 | WILSON, Rodney | JR | Jefferson City High School | 23.00 | Unverified: |
| 3 | GILES, Chris | SR | Rock Bridge High School | 22.64 | Verified |
| 4 | HOOD, Casey | JR | Rock Bridge High School | 21.57 | Verified |
| 5 | BETHUNE, David | SR | Jefferson City High School | 22.50 | Verified |
| 6 | HULL, Connor | SR | Capital City High School | 22.91 | Verified |
| 7 | WASHINGTON, Jaren | SR | Smith-Cotton High School | 23.26 | Verified |
| 8 | SMITH, Kaiden | JR | Smith-Cotton High School | 23.57 | Verified |
