| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FORD, Carolyn | JR | Rock Bridge High School | 2:22.18 | Verified |
| 2 | PARSONS, Grace | JR | Rock Bridge High School | 2:26.62 | Unverified: |
| 3 | LEIGH, Ellianna | SR | Hickman High School | 2:26.89 | Unverified: Rusty Hodge Invitational Meet |
| 4 | MORRISEY, Ava | SR | Helias Catholic High School | 2:30.35 | Verified |
| 5 | LINHARDT, Alyson | SR | Capital City High School | 2:37.37 | Verified |
| 6 | DEFEO, Gwyndolyn | SR | Capital City High School | 2:38.06 | Verified |
| 7 | KINSEY, Ella | 9 | Jefferson City High School | 2:47.14 | Verified |
| 8 | VERSLUES, Lauren | 9 | Helias Catholic High School | 2:47.72 | Verified |
| 9 | BOUCHAMA, Rayan | SR | Battle High School | 2:54.00 | Unverified: |
| 10 | HARRIS, Allison | JR | Battle High School | 2:55.00 | Unverified: |
| 11 | KUEHNER, Julie | 9 | Smith-Cotton High School | 2:56.68 | Verified |
| 12 | MCNALLY, Nora | 9 | Hickman High School | 3:01.20 | Verified |
