| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CRANE, Amelie | 9 | Rock Bridge High School | 2:38.63 | Verified |
| 2 | ZULLO, Kayla | 9 | Rock Bridge High School | 2:47.50 | Verified |
| 3 | MORRISEY, Iris | 9 | Helias Catholic High School | 2:48.53 | Verified |
| 4 | MOSS, Kierra | 9 | Jefferson City High School | 3:01.98 | Verified |
| 5 | SPELLMEYER, Jaden | 9 | Smith-Cotton High School | 3:02.84 | Verified |
| 6 | HANER, Avery | SO | Battle High School | 3:04.06 | Verified |
| 7 | GRELLNER, Frances | 9 | Helias Catholic High School | 3:05.47 | Verified |
| 8 | WILLIAMS, Ashlynn | 9 | Jefferson City High School | 3:21.66 | Verified |
| 9 | YOUNG, Sabrina | JR | Battle High School | 3:23.74 | Verified |
