| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | BROOKINS, Jaleah | 9 | Battle High School | 54.25 | Verified |
| 3 | NEWKIRK, Natali | 9 | Smith-Cotton High School | 52.53 | Verified |
| 4 | SCHONHARDT, Riley | SO | Jefferson City High School | 48.41 | Verified |
| 5 | SMITH, Caitlyn | SR | Hickman High School | 52.21 | Unverified: |
| 6 | BUTLER, Jordyn | JR | Battle High School | 52.90 | Verified |
| 7 | LUEBBERT, Beth | SR | Helias Catholic High School | 54.35 | Verified |
