| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Capital City High School | A | Casey | Harlan | Sharp | Golden | 8:18.20 | Verified |
| 2 | Helias Catholic High School | A | Ammons | Loesch | Grothoff | Kleffner | 8:48.99 | Unverified: |
| 3 | Hickman High School | A | Brinkman | Hall | Dhenyi | Mariano-Sanchez | 8:55.53 | Unverified: |
| 4 | Rock Bridge High School | A | Grubb | Tiemeyer | Dimond | Mohrland | 9:35.60 | Unverified: |
| 5 | Battle High School | A | Leavell | Ticgelaor | Webster | Tuyisenge | 10:02.23 | Verified |
| 6 | Jefferson City High School | A | Louraine | Reeves | Conger | Rukaria | 10:13.00 | Unverified: |
