| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WHITE, Alissa | 9 | Capital City High School | 1:01.09 | Verified |
| 2 | OKPODIGHE, Emerald | SO | Jefferson City High School | 55.07 | Verified |
| 3 | HILL, Carmen | 9 | Capital City High School | 54.74 | Verified |
| 4 | NEWBY, Serena | JR | Battle High School | 54.06 | Verified |
| 5 | REDCAY, Kaylee | 9 | Jefferson City High School | 54.54 | Verified |
| 6 | NUSBAUM, Eden | 9 | Hickman High School | 54.87 | Verified |
| 7 | CARTER, Ciara | SO | Battle High School | 55.99 | Verified |
