| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Helias Catholic High School | A | Lanigan | Fisher | Neuenswander | Knernschield | 45.64 | Verified |
| 3 | Battle High School | A | Holt | Rice | Horn | Ojo | DQ | Verified |
| 4 | Capital City High School | A | Harrison | Helmick | Dyer | Borde-Koufie | 44.43 | Verified |
| 5 | Jefferson City High School | A | Ice | Wilson | Bethune | Wilson | 44.54 | Verified |
| 6 | Smith-Cotton High School | A | Lorenzo | Neth | Brewer | Sims | 45.49 | Verified |
