| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Helias Catholic High School | A | Sappenfield | Campbell | Boicourt | Crisostomo | 51.64 | Unverified: |
| 2 | Battle High School | A | Weir | Murray | Harris | Carter | 48.90 | Unverified: Bill Summa Invite |
| 3 | Smith-Cotton High School | A | Washington | Staten | Blas | Brenich | 45.49 | Verified |
| 4 | Capital City High School | A | McNeil | Obi | Barnes | Otten | 44.43 | Verified |
| 5 | Jefferson City High School | A | Swanigan | Jordan | LaViolette Stewart | Parker | 45.25 | Unverified: |
| 6 | Hickman High School | A | Frederick | Stockman | Renoe | Patchett | 47.30 | Unverified: |
| 7 | Rock Bridge High School | A | Mueller | Fairlamb | Boster | Ni | 49.00 | Unverified: |
