| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Battle High School | A | May | Martin | Schultz | Carter | 58.90 | Unverified: Bill Summa Invite |
| 2 | Rock Bridge High School | A | Scott | Lockett | McCall | Dawson | 55.80 | Unverified: |
| 3 | Smith-Cotton High School | A | Bennett | Spellmeyer | Taylor | Chalfant | 54.74 | Verified |
| 4 | Capital City High School | A | Kamara | Defeo | Otten | Witt | 52.41 | Verified |
| 5 | Jefferson City High School | A | Ochoa | Okpodighe | Houston | Hearn | 54.61 | Unverified: |
| 6 | Hickman High School | A | Kennedy | Hoffman | Nusbaum | Redmond-Lilly | 55.21 | Unverified: |
| 7 | Helias Catholic High School | A | Dollar | Godbee | Moellers | Morrisey | 57.44 | Unverified: |
