| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Rock Bridge High School | A | Parsons | Sexten | Steuber | Barrett | 10:28.08 | Verified |
| 2 | Capital City High School | A | Abbott | Defeo | Linhardt | Robben | 10:52.06 | Verified |
| 3 | Helias Catholic High School | A | Lamb | Verslues | Buker | Morrisey | 11:01.30 | Verified |
| 4 | Jefferson City High School | A | Kinsey | Jones | Jobe | Cooley | 11:03.02 | Verified |
| 5 | Hickman High School | A | Domeier | Eastman | McNally | Parker | 11:04.72 | Verified |
| 6 | Smith-Cotton High School | A | Goodwin | Kuehner | Kensinger | Dmitruk | 11:24.40 | Verified |
