| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HOCKMAN, Ethan | 9 | Rock Bridge High School | 4:50.19 | Verified |
| 2 | LEE, Noah | 9 | Rock Bridge High School | 4:56.24 | Verified |
| 3 | MUHLENBRUCK, Elliot | JR | Hickman High School | 4:58.57 | Verified |
| 4 | TICGELAOR, Ben | SO | Battle High School | 5:23.35 | Verified |
| 5 | WALKER, Gauge | SO | Capital City High School | 5:29.17 | Verified |
| 6 | WHITAKER, Tyren | SO | Capital City High School | 5:32.45 | Verified |
| 7 | GROTHOFF, Samuel | 9 | Helias Catholic High School | 5:36.93 | Verified |
| 8 | SELTZER, Dominic | JR | Smith-Cotton High School | 5:45.00 | Verified |
| 9 | TICGELAOR, Alex | SO | Battle High School | 5:56.02 | Verified |
| 10 | WIEBERG, Jonathan | JR | Helias Catholic High School | 5:56.78 | Verified |
| 11 | CONGER, Joshua | SO | Jefferson City High School | 5:57.05 | Verified |
| 12 | MARIANO-SANCHEZ, Victor | 9 | Hickman High School | NT | Unverified: |
