| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FORD, Carolyn | JR | Rock Bridge High School | 5:13.97 | Verified |
| 2 | LEIGH, Ellianna | SR | Hickman High School | 5:23.80 | Unverified: Rusty Hodge Invitational Meet |
| 3 | WALKER, Brooke | SR | Rock Bridge High School | 5:33.56 | Unverified: |
| 4 | ROBBEN, Yumia | JR | Capital City High School | 5:45.30 | Verified |
| 5 | BUKER, Danielle | JR | Helias Catholic High School | 5:50.09 | Unverified: |
| 6 | LAMB, Alexa | SR | Helias Catholic High School | 5:50.17 | Verified |
| 7 | GOODWIN, Leia | SR | Smith-Cotton High School | 6:01.84 | Verified |
| 8 | KIRBY, Sarah | JR | Capital City High School | 6:04.03 | Verified |
| 9 | COOLEY, Hayli | 9 | Jefferson City High School | 6:11.40 | Verified |
| 10 | REYES, Josefina | SR | Smith-Cotton High School | 6:30.70 | Verified |
| 11 | HARRIS, Allison | JR | Battle High School | 6:38.00 | Unverified: |
| 12 | BOUCHAMA, Rayan | SR | Battle High School | NT | Unverified: |
