| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FISHER, Grace | SR | Rock Bridge High School | 5:42.02 | Verified |
| 2 | MATHEWS, Lauren | 9 | Helias Catholic High School | 6:18.16 | Verified |
| 3 | BLACKBURN, Bailey | SO | Rock Bridge High School | 6:19.15 | Verified |
| 4 | GRELLNER, Frances | 9 | Helias Catholic High School | 6:47.75 | Verified |
| 5 | WILLIAMS, Ashlynn | 9 | Jefferson City High School | 7:17.99 | Verified |
