| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Battle High School | A | Owusu | Rice | Karara | Anderson | DQ | Verified |
| 2 | Jefferson City High School | A | Bethune | Wilson | Wilson | Wilson | DQ | Verified |
| 3 | Helias Catholic High School | A | Lanigan | Fisher | Wilson | Malmstrom | 1:33.66 | Verified |
| 4 | Rock Bridge High School | A | Giles | Allen | Seamon | Hood | DQ | Verified |
| 5 | Capital City High School | A | Harrison | Hull | Dyer | Grieve | 1:31.40 | Verified |
| 6 | Smith-Cotton High School | A | Smith | Washington | Grubb | Jaekel | 1:34.74 | Verified |
| 7 | Hickman High School | A | Milo | McNally | Taylor | Holt | 1:35.14 | Verified |
