| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Battle High School | A | Weir | Murray | Carter | Nelson | DNF | Unverified: Darwin Rold Invitational |
| 2 | Rock Bridge High School | A | Rolwing | Bailey | Rychnovsky | Mueller | 1:38.50 | Unverified: |
| 3 | Hickman High School | A | Frederick | Renoe | Watson | Gould | 1:35.64 | Unverified: 62nd LickLider Invite |
| 4 | Capital City High School | A | Obi | McNeil | Otten | Gant | 1:31.40 | Verified |
| 5 | Smith-Cotton High School | A | Washington | Staten | Blas | Brenich | 1:34.74 | Verified |
| 6 | Jefferson City High School | A | Swanigan | Jordan | LaViolette Stewart | Parker | 1:36.78 | Unverified: |
| 7 | Helias Catholic High School | A | Boicourt | Schatzer | Sappenfield | Crisostomo | 1:43.66 | Unverified: |
