| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Capital City High School | A | Sterrett | Cris-Kargbo | York | Strayhorn | 1:55.17 | Verified |
| 3 | Jefferson City High School | A | Dunbar | Morris | Hearn | Galbreath | 1:50.91 | Verified |
| 4 | Rock Bridge High School | A | Porter | Miller | Crane | Nunnelly | 1:47.86 | Verified |
| 5 | Helias Catholic High School | A | Phillips | Struemph | Harrison | Edwards | 1:48.15 | Verified |
| 6 | Hickman High School | A | Peterson | Leigh | Orr | Stemmons | 1:51.12 | Verified |
| 7 | Smith-Cotton High School | A | Robinson | Simmons | Newkirk | Williams | 2:02.88 | Verified |
