| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Rock Bridge High School | A | Lockett | Walker | Goeckler | McCall | 2:02.50 | Unverified: |
| 3 | Jefferson City High School | A | Hearn | Woodard | Houston | Okpodighe | 1:57.68 | Unverified: |
| 4 | Hickman High School | A | Redmond-Lilly | Goodman | Coleman | Monnig | 1:55.12 | Unverified: |
| 5 | Capital City High School | A | Kamara | White | Thompson | Defeo | 1:55.17 | Verified |
| 6 | Helias Catholic High School | A | Dollar | Godbee | Kleene | Moellers | 1:58.15 | Unverified: |
