| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Licking High School | A | Donley | Richards | Corkell | Mendenhall | 1:53.36 | Verified |
| 2 | Bourbon High School | A | Reeves | Politte | Pietraschke | Reeves | 1:44.09 | Verified |
| 3 | St. Paul Lutheran (Farmington) | A | Anderson | Jordan | Peak | Roberson | 1:40.72 | Verified |
| 4 | Sullivan High School | A | Rall | Montee | Goly | Hecht | 1:37.06 | Verified |
| 5 | Steelville High School | A | Harris | Brown | Killeen | Brown | 1:39.78 | Verified |
| 6 | Cuba High School | A | Nelson | DeClue | Hinojosa | Bonney | 1:42.33 | Verified |
| 7 | Viburnum High School | A | Hardy | Zundell | Murphy | Hubbs | NT | Verified |
