| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Paul Lutheran (Farmington) | A | Blackmon | 2:16.14 | Verified | |||
| 3 | Viburnum High School | A | Pyatt | Halbert | Barker | Wigger | 2:03.37 | Verified |
| 4 | Bourbon High School | A | Clonts | Poole | Gebhart | Enke | 2:01.21 | Verified |
| 5 | Steelville High School | A | Perkins | Kreitner | Davis | Pryor | 2:02.32 | Verified |
| 6 | Cuba High School | A | Carlos-Alejo | Lunsford | Soppeland | West | 2:07.82 | Verified |
| 7 | Cuba High School | B | NT |
