| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Cuba High School | A | Fulliam | Ijames | Moreland | Deluna | 5:08.69 | Verified |
| 4 | Steelville High School | A | Kreitner | Davis | Diaz | Pryor | 4:37.21 | Verified |
| 5 | St. Paul Lutheran (Farmington) | A | 5:05.50 | Verified | ||||
| 6 | Newburg High School | A | Caquelin | Harris | Logan | Twyman | 5:15.76 | Unverified: |
| 7 | Cuba High School | B | NT |
