| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | MCLAIN-WILLIAMS, Alexandra | SO | Sullivan High School | NT | Unverified: |
| 3 | CARLOS-ALEJO, Ana | JR | Cuba High School | 36.80 | Verified |
| 4 | BOGERT, Loghan | JR | Sullivan High School | 34.49 | Verified |
| 5 | BAER, Anna | 9 | St. Paul Lutheran (Farmington) | 36.55 | Verified |
| 6 | SOPPELAND, Tanya | 9 | Cuba High School | 37.83 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MCCARTY, Taylor | SO | St. Paul Lutheran (Farmington) | 33.12 | Unverified: Saxony Last Chance |
| 2 | MACE, Jaycie | SO | Newburg High School | 32.71 | Verified |
| 3 | LUNSFORD, Ciara | SO | Cuba High School | 31.98 | Verified |
| 4 | CLONTS, Cierra | SO | Bourbon High School | 31.25 | Verified |
| 5 | PYATT, Lanee | SO | Viburnum High School | 31.61 | Verified |
| 6 | HALBERT, Natalie | 9 | Viburnum High School | 32.27 | Verified |
| 7 | MEDLING, Kennedy | 9 | Sullivan High School | 32.78 | Verified |
| 8 | GOVERO, Valerie | 9 | St. Paul Lutheran (Farmington) | 33.34 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | POOLE, Abigail | 9 | Bourbon High School | 30.49 | Verified |
| 2 | HARRIS, Kaliethia | JR | Newburg High School | 30.01 | Verified |
| 3 | BARKER, Alexus | SR | Viburnum High School | 29.26 | Verified |
| 4 | SULLINS, Finley | SO | Licking High School | 28.84 | Verified |
| 5 | ENKE, Marisa | 9 | Bourbon High School | 28.91 | Verified |
| 6 | PRYOR, Daylan | JR | Steelville High School | 29.81 | Verified |
| 7 | DAVIS, Lauren | SR | Steelville High School | 30.27 | Verified |
| 8 | PERKINS, Marli | SO | Steelville High School | 30.84 | Verified |
