| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DIAZ, Conner | JR | Steelville High School | 2:00.59 | Verified |
| 2 | HUFF, Jarrett | SR | Licking High School | 2:16.05 | Verified |
| 3 | CROCKER, Vincent | 9 | Steelville High School | 2:16.77 | Verified |
| 4 | PEAK, Brett | JR | St. Paul Lutheran (Farmington) | 2:28.59 | Verified |
| 5 | ZUNDELL, Kameron | 9 | Viburnum High School | 2:39.05 | Verified |
| 6 | MELTON, Ryan | SR | Licking High School | 2:39.91 | Verified |
| 7 | MERTZ, Mason | 9 | Sullivan High School | 2:46.64 | Verified |
| 8 | HARRIS, Tyson | SO | Steelville High School | 2:48.36 | Verified |
| 9 | SCHLUETER, Logan | 9 | Sullivan High School | 2:49.65 | Verified |
| 10 | WHALEY, Parker | SO | Sullivan High School | 2:56.97 | Verified |
| 11 | MASSEY, John | JR | Cuba High School | 3:07.50 | Verified |
| 12 | STEVENSON, Tyler | SR | Newburg High School | 4:19.20 | Verified |
