| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 4 | BUSSELL, Allyson | JR | Cuba High School | 1:18.85 | Verified |
| 5 | KELLY, Bre | 9 | Steelville High School | 1:19.35 | Verified |
| 6 | MACE, Jaycie | SO | Newburg High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | IJAMES, Izzabelle | SO | Cuba High School | 1:16.77 | Verified |
| 2 | GEBHART, Mia | SO | Bourbon High School | 1:12.49 | Verified |
| 3 | CLONTS, Cierra | SO | Bourbon High School | 1:09.78 | Verified |
| 4 | PRYOR, Daylan | JR | Steelville High School | 1:05.14 | Verified |
| 5 | BARKER, Alexus | SR | Viburnum High School | 1:05.55 | Verified |
| 6 | CONAWAY, Jorja | SO | Viburnum High School | 1:11.81 | Verified |
| 7 | EVANS, Norah | SO | Steelville High School | 1:14.06 | Verified |
| 8 | MCCARTY, Taylor | SO | St. Paul Lutheran (Farmington) | 1:17.93 | Verified |
