| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Paul Lutheran (Farmington) | A | Anderson | Dumas | Garner | Folk | 51.99 | Verified |
| 3 | Steelville High School | A | Killeen | Harris | Brown | Harris | 48.40 | Verified |
| 4 | Sullivan High School | A | Goly | Hecht | Montee | Rall | 46.30 | Verified |
| 5 | Cuba High School | A | Dake | Declue | Hinojosa | Volner | 48.37 | Verified |
| 6 | Licking High School | A | Bartling | Corkell | Richards | Donley | 50.09 | Verified |
| 7 | Cuba High School | B | NT |
