| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Licking High School | A | Hock | Murray | Sullins | Richards | 58.40 | Verified |
| 4 | Steelville High School | A | Davis | Perkins | Perkins | Kreitner | 55.61 | Verified |
| 5 | Cuba High School | A | Dong | Moreland | Martellaro | Deluna | 58.03 | Verified |
| 6 | St. Paul Lutheran (Farmington) | A | 1:02.86 | Verified | ||||
| 7 | Cuba High School | B | NT |
