| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | New Haven High School | A | Steele | Meyer | Strubberg | Groner | DQ | Unverified: |
| 3 | Wright City High School | A | NT | Unverified: | ||||
| 4 | Helias Catholic High School | A | Phillips | Ehmke | Struemph | Edwards | 1:49.94 | Verified |
| 5 | Helias Catholic High School | B | Harrison | Moellers | Dollar | Morrisey | 1:49.94 | Verified |
| 6 | Wright City High School | B | NT | Unverified: |
