| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Sullivan High School | A | Mayberry | Smith | Halmick | Halmick | NT | Unverified: |
| 4 | Helias Catholic High School | A | Luebbert | Verslues | Ehmke | Harrison | 4:39.10 | Verified |
| 5 | New Haven High School | A | Steele | Luecke | Groner | Strubberg | NT | Unverified: |
| 6 | Wright City High School | A | NT | Unverified: |
