| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | MCCLAIN, Daveah | 9 | Sullivan High School | NT | Unverified: |
| 4 | VO, Linh | SO | Wright City High School | NT | Unverified: |
| 5 | SMITH, Grace | 9 | St. Francis Borgia High School | NT | Unverified: |
| 6 | RICHARDSON, Audrey | SR | St. Francis Borgia High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PETTUS, Lindsay | 9 | Wright City High School | NT | Unverified: |
| 2 | MAYER, Kersten | 9 | St. Francis Borgia High School | NT | Unverified: |
| 3 | VOLKERT, Ashley | SR | Sullivan High School | NT | Unverified: |
| 4 | CUPP, Ann | JR | Sullivan High School | 1:29.03 | Verified |
| 5 | DALTON, Dakota | 9 | Wright City High School | NT | Unverified: |
| 6 | CANNON, Brooklyn | 9 | St. Clair High School | NT | Unverified: |
| 7 | NUNEZ-CRUZ, Nicole | 9 | Wright City High School | NT | Unverified: |
| 8 | STEPHENS, Lauryn | 9 | Wright City High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CARPENTER, Makenzie | SO | Sullivan High School | 1:18.50 | Verified |
| 2 | MURRAY, Alexis | SO | St. Clair High School | 1:13.13 | Verified |
| 3 | MANTHEY, Allison | SR | Salem High School | 1:08.12 | Verified |
| 4 | EDWARDS, Mikah | JR | Helias Catholic High School | 57.77 | Verified |
| 5 | BRAND, Ava | JR | St. Clair High School | 1:05.44 | Verified |
| 6 | KLEENE, Allie | SR | Helias Catholic High School | 1:08.25 | Verified |
| 7 | MILLER, Melodi | JR | St. Clair High School | 1:16.16 | Verified |
| 8 | CABANLIT, Zandra | JR | Sullivan High School | 1:24.02 | Verified |
