| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Wright City High School | A | NT | Unverified: | ||||
| 3 | Sullivan High School | A | Bogert | Cabanlit | Carpenter | McLain-Williams | 1:03.46 | Verified |
| 4 | Helias Catholic High School | B | Dollar | Moellers | Godbee | Schmidt | 52.12 | Verified |
| 5 | Helias Catholic High School | A | Struemph | Ehmke | Harrison | Edwards | 52.12 | Verified |
| 6 | New Haven High School | A | Sumpter | Meyer | Strubberg | Groner | NT | Unverified: |
| 7 | Wright City High School | B | NT | Unverified: |
