| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | NEISEN, Arianna | SO | Liberty | 5:30.12 | Unverified: |
| 2 | SCHUERING, Olivia | SO | Quincy (Sr.) | 5:35.76 | Verified |
| 3 | DORSEY, Jocelyn | SO | Hannibal High School | 5:36.95 | Verified |
| 4 | MCCLELLAND, Amick | 9 | Quincy (Sr.) | 5:41.68 | Verified |
| 5 | OBRIEN, Jerorgia | JR | Palmyra High School | 5:59.40 | Verified |
| 6 | WYMAN, Emma | SO | Jacksonville (H.S.) | 5:59.85 | Verified |
| 7 | ELLIS, Matera | JR | Mark Twain High School | 6:01.00 | Verified |
| 8 | NOLAND, Alyssa | JR | Palmyra High School | 6:09.01 | Verified |
| 9 | HART, Anna | SO | Ft. Zumwalt South High School | 6:16.69 | Verified |
| 10 | WHITAKER, Emma | SO | Carthage (Illini West) | 6:18.91 | Verified |
| 11 | LYONS, Peyton | SR | Rushville (R.-Industry) | 6:23.88 | Verified |
| 12 | BONVILLIAN, Ava | 9 | Hannibal High School | 6:26.32 | Verified |
| 13 | RESOR, Cynthia | SO | Mark Twain High School | 6:28.75 | Verified |
| 14 | BIBB, Dianna | 9 | Bowling Green High School | 6:32.51 | Verified |
| 15 | STERUP, Evangeline | 9 | Kirksville High School | 6:34.18 | Verified |
| 16 | ZOELLNER, Julia | SO | Jacksonville (H.S.) | 6:35.00 | Unverified: |
| 17 | WADDELL, Abbie | SR | Bowling Green High School | 6:36.99 | Verified |
| 18 | GADDY, Allison | SO | Ft. Zumwalt South High School | 7:04.86 | Verified |
| 19 | MELNICK, Tyne | 9 | Kirksville High School | 7:12.41 | Verified |
| 20 | COOK, Addison | 9 | Carthage (Illini West) | NT | Unverified: |
