| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Quincy (Sr.) | A | Bryan | Bruening | Emeka | Moore | 1:43.38 | Unverified: |
| 2 | Mark Twain High School | A | Lake | Zumwalt | Lake | Miller | 1:40.85 | Verified |
| 3 | South Shelby High School | A | Moellering | Elsen | Wiseman | Hammond | 1:38.18 | Verified |
| 4 | Kirksville High School | A | Farwell | Mendez | Novencido | Austic | 1:40.55 | Verified |
| 5 | Palmyra High School | A | Getz | Pennewell | Dames | Smyser | 1:41.82 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Jacksonville (H.S.) | A | Wright | Johnson | Range | Mitchell | 1:32.63 | Verified |
| 3 | Hannibal High School | A | Robertson | Bergheger | Williams | Humphrey | 1:31.11 | Verified |
| 4 | Ft. Zumwalt North High School | A | Miller | Lee | Weddington | Reckel | 1:31.43 | Verified |
| 5 | Bowling Green High School | A | Bryant | Dalton | Gamm | Starks | 1:33.31 | Verified |
| 6 | Pittsfield | A | Pherigo | Ballinger | Petty | Poor | 1:36.05 | Verified |
