| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Pittsfield | A | Veile | Salter | Olson | Lawson | NT | Unverified: |
| 2 | Bowling Green High School | A | Mix | Hearn | Jennings | Gamm | 3:48.02 | Verified |
| 3 | Quincy (Sr.) | A | Tipton-hicks | Savage | Mcclelland | Homan | 3:41.00 | Unverified: |
| 4 | Palmyra High School | A | Bogue | Dames | Smyser | Miller | 3:41.86 | Verified |
| 5 | Liberty | A | 3:50.89 | Unverified: | ||||
| 6 | Jacksonville (H.S.) | A | Wright | Finn | Neice | Range | NT | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Hannibal High School | A | Humphrey | Nichols | Williams | Bergheger | 3:34.02 | Verified |
| 3 | Ft. Zumwalt North High School | A | Miller | Lee | Mason | Reckel | 3:31.70 | Verified |
| 4 | Kirksville High School | A | Harvey | Krepps | Amerman | Crooks | 3:33.94 | Verified |
| 5 | Ft. Zumwalt South High School | A | Bates | Bass | Dewitt | Raife | 3:37.42 | Verified |
| 6 | Mark Twain High School | A | Preston | Northcutt | Owen | McLeod | 3:39.69 | Verified |
